The p2.1 PF-00562271 and GalA assays revealed that double mutation of Pro-403/408 significantly reduced PKM2-mediated HIF-1 transactivation in HeLa cells (Figures 4F and 4G), despite the fact that PKM2(P403/408A)-V5 was detected in the nucleus at levels similar to WT PKM2-V5 (Figure S3C). demonstrate PKM2 hydroxylation on proline-403/408. PHD3 knockdown inhibits PKM2 coactivator function, reduces glucose … Continue reading The p2